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June 23, 2003 9:39 am, MFC_QSG_text.fm
Preparing and Running the Micro Fluidic Cards (PCR)
Applied Biosystems Micro Fluidic Card Getting Started Guide 21
About Micro Fluidic Card Templates
SDS template documents (*.sdt) contain the same information as
SDS documents, but do not store raw data. You can use templates to
create duplicate plate documents for a series of plates with identical
configurations.
Using the SDS Setup File to Generate SDS Templates
When you order a custom Micro Fluidic Card, the accompanying
Micro Fluidic Card Information CD contains an SDS setup file. The
setup file contains the following information that is specific to your
custom Micro Fluidic Card:
• Detector – Indicates the gene symbol and Applied Biosystems
assay number
• Reporter – Indicates the reporter dye
• Task – Indicates the task of the well (Endogenous Control,
Target, Unknown, Standard, or NTC)
Instead of entering the above information manually for each assay,
SDS software allows you to import the information from the setup
file, saving time and effort. You can then save the file as an SDS
document (*.sds) or an SDS template (*.sdt).
Note: Appendix A contains additional information about the SDS
setup file and other contents of the Micro Fluidic Card Information
CD.
The following procedure explains how to use the SDS setup file to
create a Micro Fluidic Card template or document for relative
quantification.
Note: The procedure assumes that you have calibrated the 7900HT
system by performing both background and pure dye runs for the
Micro Fluidic Card. For more information about calibration, see
Chapter 7 of the user guide for the 7900HT system.
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