Page 56 of 59 BACTcontrol – User Manual – V0.4.4 microLAN b.v.
In the reaction chamber the enzyme comes in contact with the substrate. As shown in
the figure 33 above, the enzymes attach to the substrate previous to the conversion,
meaning that the enzyme can convert one molecule substrate at the time. Enzymatic
activity is expressed as the amount fluorescent substrate that is produced (REF. 3).
When a substrate molecule is converted, the enzyme goes further with the next
substrate molecule. In the first stage of the measurement, the signal will increase
gradually therefore a stabilization period is necessary. When the signal is stable, then
the conversion of measured fluorescence to specific enzymatic activity takes place. In
the BACTcontrol, enzymatic activity is expressed in picomole per minute.
Figure 33 General working of enzymes
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