Chapter 7: Technical overview 103
FITC have a smaller Stokes shift, absorbing blue light (488 nm)
and emitting green light (530
nm).
The following figure shows the emission spectra of some
commonly used fluorochromes.
Actual emission intensity will depend on excitation wavelength.
See
Fluorescence spectra (page 128) for more information on
excitation and emission of fluorochromes. An interactive spectral
viewer is also available at
bdbiosciences.com.
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