100-214-239 Rev. A A-13
Appendix A
Digital Sonifier
Skin:
1 gm disintegrates in about 4 minutes. Epidermal homogenates that respire and utilize
substrate can be extracted.
Soil:
separates solid particles without use of oxidants, acids or peptizing agents and yields
stable suspensions.
Sperm (human):
tails are broken instantly. Heads are broken in 20 minutes.
Sputum:
cancer cells are more easily detected after ultrasonic treatment due to even
dispersion of cells and sputum, and complete liberation of the cells from sputum.
Staphylococcus:
a concentrated solution disrupts 98% in 10 minutes. With 1 gm cells wet wt,
to 2 gm water, 54.5 mg/ml of protein was released.
Starch:
obtained by extracting from green plant leaf homogenate.
Streptococcus, Group A:
a 20% suspension in a 15 ml solution completely disr upts in 15
minutes.
Streptomyces:
monocellular elements from surface-grown colonies obtained in 1 minute.
Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Subcellular particles:
may be separated or broken depending on power selection and length
of time.
Sulfanilamide:
excellent dispersion in less than 1 minute. Continued ultrasonic treatment will
produce complete disr uption.
Sulfapyridine:
excellent dispersion in less than 1 minute. Continued ultrasonic treatment will
produce complete disr uption.
Synovial fluid:
ultrasonic treatment is an excellent means of reducing fluid viscosity. It is sim-
pler and faster than using hyaluronidase.
T. pyriformis:
excellent disruption, 8 enzymes released.
Tablets:
complete disruption in 2-40 seconds, depending on type.
Tea:
excellent extraction.
Tetrahymena:
disrupts in a few seconds. Enzymes which have been monitored include:
succinate, lactate, B-hydroxy butyrate, glutamate and DPNH oxidases,
DPNH-cytochromeC , reductase and ribonuclease. Activity of DPNH oxidase was twice that
of the best previous experiments.
Themoactinomyces:
disruption of hyphae. Homogenization of protein complex without
denaturation.
Thermophile negative:
good disruption within 2 minutes.
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