Application Information
A-14 100-214-239 Rev. A
Thermophilic bacillus:
isocitrate lyase was extracted from a spore-forming bacillus similar
to Stearothermophilus. A washed cell paste suspended in a phosphate buffer was sonified 1-2
minutes and the supernatent was used for enzyme experiments without further
treatment. Extracts could be frozen and stored without loss of activity.
Thiouric acid:
dissolved in a few seconds.
Thymus cells:
complete disruption in 15 seconds.
Tissue culture cells:
complete disruption in a few seconds. To avoid damage to free
organelles and to obtain intact lysosomes, use low power at short exposure.
Toxin and antitoxin:
one example of many: Toxin preparations of whole cell lysate (WCL) of
the Inaba serotype strain 569E of the classic biotype of cholera vibrio were grown on 3% Bacto
peptane agar and harvested in distilled water at 18 hours. The unwashed suspensions were sol-
ubilized ultrasonically, clarified by centrifugation and the supernate freeze-dried for the titra-
tion of cholare toxin in the rabbit ileal loop.
Toxoplasma gondii:
can be separated form white blood cells without injury.
Transplantation antigens:
were extracted from spleen, thymus and lymph nodes.
Trichomonas foetus:
complete disruption in a few seconds.
Triolei n:
complete stable emulsion in 2 minutes.
Trypanosomes:
concentrated 10 ml solution completely disrupts in 4 minutes.
Uterus muscle:
a 1/5 gm, 3 cc solution completely disrupts in 3 minutes for coenzyme Q
determination.
Vaccines:
numerous advantages, such as more antigenic material released than usual, and the
production of vaccines not obtainable by classified methods.
Various ba cilli:
complete disruption in 3 minutes.
Vibrio comma:
excellent disr uption.
Vibrio extraction:
excellent for experimental vaccines. Evidence of breakage of virus/anti-
body bonds. Virus can be extracted without damage at low power, or broken at high power.
Vitamin E:
30 seconds of ultrasonic treatment put material in solution with a resultant
permanent suspension.
W138 virus:
cell-free V-2 virus obtained in 30 seconds using 6 m l of Veronal buffer with W138
cells containing V-2 virus.
Yeast:
complete disruption in 3-10 minutes. Complete disruption of 9 grams pressed yeast in
18 ml buffer in 8 minutes. Protein release, 52 mg/ml from an aged sample.
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