Dionex UltiMate 3000 - Page 62

UltiMate 3000 Nano and Cap- System Installation and Application
58
Problem Probable Cause Remedial Action
High noise level, pulsation There are pressure fluctuations
from the pump.
Prime the pump; check general
function (→ Pump manual).
There are gas bubbles in the
system.
Prime the system
(→ Pump manual).
The solvent is degraded. Use fresh solvent.
The detector is defective. Contact Dionex Service.
The lamp is too old. Replace the detector lamp.
Peak broadening,
increased dead time
The capillary's inner diameter is
too large.
Change the capillary.
The filter frits on the solvent
lines are clogged.
Check the filter for permeability.
Replace the filter frit if necessary
(→ Pump manual).
The capillaries are clogged. Replace the capillaries.
The sample loop is clogged. Replace the sample loop
(→ Autosampler manual).
The proportioning valve is
defective.
Contact Dionex Service.
The column is overloaded or
contaminated.
Clean or replace the column.
The solvent is degraded. Use fresh solvent.
The flow splitter is clogged or
defective.
Replace the splitter cartridge
(→ Flow Manager manual). If
this does not solve the problem,
contact Dionex Service.
Triangular peaks The column is overloaded (the
protein concentration is too
high).
Dilute the sample.
Additional peaks appear in
the injection peak.
With gradients, the equilibration
time after the flush cycle is too
short or the dead volume is too
high.
Increase the equilibration time
and/or eliminate possibly existing
dead volume.
Poor reproducibility The autosampler draws air from
the vial.
There is not enough sample in the
vial, the settings for the
SampleHeight and/or
TransLiquidHeight autosampler
parameters are incorrect, or there
are too many replicates
(→ Operating Instructions for
the autosampler).
There are gas bubbles in the
syringe.
Flush the syringe
(→ Autosampler manual).
There is a gas bubble in the flow
path.
Perform a wash cycle
(→ Autosampler manual).
There is a gas bubble at the
bottom of the vial.
Remove gas bubble from vial.
The autosampler, the injection
valve, or the syringe valve is not
tight.
→ Autosampler manual