UltiMate 3000 Nano and Cap- System Installation and Application
20
3.2 Direct Injection
For this application, sample is drawn by the syringe through the needle and placed in the
sample loop when the autosampler's injection valve is in Load position. The needle pair
withdraws liquid from the well or vial and then descends into the wash port. The injection
valve is switched to Inject position, directing the sample flow from the sample loop through
the high-pressure circuit to the separation column.
Dionex recommends that you stack the system modules as shown below:
Fig. 8: Module arrangement for direct injection
The system includes the modules listed in the table below. In addition, the appropriate
Direction Injection Kit (→ section 8.2, page 64) is required.
Part No. System Module
Nano HPLC Capillary HPLC Micro HPLC Monolith. HPLC
SR-3000 5035.9200
LPG-3400 5035.0045 (standard) or 5037.0055 (biocompatible)
VWD-3100*
VWD-3400*
5074.0005
5074.0010
*Please note that flow cells must be ordered separately, for order numbers see page 15.
FLM-3300
Standard
Biocompatible
5720.0030
5721.0030
5720.0035
5721.0035
5720.0038
5721.0038
5720.0035
5721.0035
WPS-3000 or
WPS-3000 T
5820.0010 (standard) or 5821.0010 (biocompatible)
5820.0020 (standard) or 5821.0010 (biocompatible)
Direct Injection Kit
Standard
Biocompatible
6720.0042
6721.0042
6720.0043
6721.0043
6720.0046
6721.0046
6720.0044
6721.0044
SR-3000 Solvent Rack
LPG-3400 Pump
VWD-3x00 Detector
FLM-3300 Flow Manager
WPS-3000 or WPS-3000T Autosampler
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