UltiMate 3000 Nano and Cap- System Installation and Application
24
3.3 Preconcentration
For this application, sample is drawn by the syringe through the needle and placed in the
sample loop when the autosampler's injection valve is in Load position. The needle pair
withdraws liquid from the well or vial and then descends into the wash port. The injection
valve is switched to the Inject position, allowing the loading pump to direct the sample flow
from the sample loop onto the trap column for sample enrichment. (For information about
how to install the trap column, refer to section 3.7.) The switching valve in the flow manager
is switched from 10-1 to 1-2. The micro pump and the flow splitter direct the sample onto the
separation column. Dionex recommends that you stack the system modules as shown below:
Fig. 11: Module arrangement for preconcentration
The system includes the modules listed in the table below. In addition, the appropriate
Preconcentration Kit (→ section 8.3, page 65) is required:
Part No. System Module
Nano HPLC Capillary HPLC Micro HPLC Monolith. HPLC
SRD-3600 5035.9230
DGP-3600 5035.0050 (standard) or 5037.0060 (biocompatible)
VWD-3100*
VWD-3400*
5074.0005
5074.0010
*Please note that flow cells must be ordered separately, for order numbers see page 15.
FLM-3300
Standard
Biocompatible
5720.0030
5721.0030
5720.0035
5721.0035
5720.0038
5721.0038
5720.0035
5721.0035
WPS-3000 or
WPS-3000 T
5820.0010 (standard) or 5821.0010 (biocompatible)
5820.0020 (standard) or 5821.0020 (biocompatible)
Preconcentration Kit
Standard
Biocompatible
6720.0047
6721.0047
6720.0048
6721.0048
6720.0050
6721.0050
6720.0049
6721.0049
SRD-3600 Solvent Rack
DGP-3600 Pump
VWD-3x00 Detector
FLM-3300 Flow Manager
WPS-3000 or WPS-3000T Autosampler
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