36
PC materials are low in chemical resistance against alkaline solutions. Avoid using
neutral detergents with pH higher than 9. Note that pH of some neutral detergents
are still higher than 9 even if diluted according to the instruction in the maker‘s
catalog. Use detergent with its pH between 7 and 9.
11.2.3AutoclavingPA、PC and PP tubes
PA begins softening at about 120℃, PC and PP at about 130℃. Autoclave PA tubes
at 115℃(0.7kg/cm 2)for 30 minutes and PC and PP tubes at 121℃(0.1kg/cm
2)for 20 minutes. If a certain temperature is exceeded, the tubes may be deformed.
When using a sterilizing chamber, please operate as follows:
(1)Place tubes in vertical position, mouths upward. If tubes are placed sideways,
they may deform into an oval shape due to gravity.
(2)Remove screw nuts and inner covers to prevent from deformation or rupture.
(3)Wait until the sterilizing chamber cools down to the room temperature before
the tubes are removed.
11.2.4Condition and life expectancy of tubes
The life expectancy of plastic tubes depends on the characteristics of samples,
speed of the rotor used, and temperature applied, and so on. When the plastic
tubes are used for centrifuge of ordinary aqueous samples (pH between 5 and 9),