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SECTION 3: SWEAT INDUCTION AND COLLECTION
3.1 Preparing for Sweat Induction
8. Install a Pilogel Disc on Red Electrode and Attach to Limb
• Before using a Pilogel disc, inspect it for cracks, fractures,
crumbling, shrinkage in size, bacterial growth, mold, or other signs
of damage. Discard any damaged discs. Verify that the Pilogel disc
is within the expiration date.
• The positive (red) electrode must be placed correctly for
successful sweat collection. Locate it on an area of skin with a
high density of sweat glands for optimum sweat yield. The
preferred site is the lower portion of the flexor aspect of the
forearm. This generally has a very high density of sweat glands,
provided the limb is not so small as to prevent proper attachment
of the Macroduct Advanced Sweat Collector.
NOTE: Do not place the electrode so close to the wrist that tendons or
bone are palpable just beneath the skin. Reasonably thick musculature is
necessary for a proper interface with the Macroduct Advanced Sweat
Collector.
• If the limb is tiny, place the red electrode on the upper portion of
the flexor aspect of the forearm (nearer the elbow) or even the
upper arm. If the entire arm is too small to attach the electrode,
use the inner thigh, placing the red electrode on the inner thigh
and the black electrode on the calf. In this case, constrain the
infant from flexing the knee to avoid a loss of interface between
the skin and the collector.
WARNING!
Never attach an electrode to the skin without a Pilogel disc. Direct
electrode-to skin contact will burn the patient. Refer to Section 1.5 for
additional information.
WARNING!
Never place electrodes across the chest or on opposite limbs. Even
though the DC iontophoretic current is extremely low, there is a remote
risk of interference with cardiac rhythms.
a. Attach the strap to one side of the red electrode by inserting the
strap from below the electrode and up through the slot. Align one
of the holes in the strap with the protruding attachment knob.
Press the hole in the strap down over the knob to lock it in place.
Attach strap to one side of the
electrode as shown.