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SECTION 3: SWEAT INDUCTION AND COLLECTION
3.3 Collecting Sweat
2. Attach Collector
a. Place the concave surface of the collector precisely over the area
of skin contacted by the Pilogel disc that was under the red
electrode.
b. While applying slight pressure to the collector, wrap the strap
around the limb and thread it up through the slot on the opposite
side of the collector. Pull the free end out and then down, aligning
a suitable hole in the strap with the attachment knob. Press the
selected hole in the strap down over the knob to lock it in place.
c. Verify that the collector is firmly attached.
d. If necessary, grip the collector and lift it briefly above the skin to
equalize strap tension on each side of the collector. Then lower
the collector to the skin surface, ensuring that the collector is still
positioned over the sweat-stimulated area. Adjust strap tension
on either side, as needed, to ensure even contact.
e. For neonate sweat collections where the limbs are extremely
small: Overwrap the collector firmly with a 5-8 cm (2 or 3 inch)
wide elastic bandage. This ensures continuous and firm contact
between the collector and the skin, and greatly improves the
probability of successful collection.
WARNING!
Watch for any signs of interference with blood circulation in the limb,
such as cyanosis, swelling, or unusual pallor, and discontinue the test on
that limb if any of those conditions should occur.
f. Tap to advance to the next screen, or tap to go back to the
previous screen.
CAUTION:
Dropping the collector or handling it improperly may cause
contamination. The collector should be discarded if the collector surface
is touched, dropped, or comes in contact with another surface.
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