Operating Instructions Raman Rxn2
36 Endress+Hauser
5. Specify names for each probe/quadrant such as Probe 1, Probe 2:
• From the dashboard, click the title bar of the probe you wish to name. The stream or probe detail view
displays.
• Select the Settings Tab and click Name.
• Enter the name of the probe and click Apply.
• Let the system stabilize for at least two hours before proceeding to calibration.
6. Refer to the Raman RunTime Operating Instructions (BA02180C) for initial calibration and verification
instructions.
8.3 Calibration and verification
Reliable, transferable calibration is important for comparing data acquired at various times or with different
analyzers. Different instruments analyzing the same sample can generate nearly identical spectra if they are properly
calibrated. The Raman RunTime software package includes an automatic calibration wizard that guides you through
a procedure to automatically calibrate the wavelength and intensity axes and the wavelength of the laser.
After the initial calibration during installation, the Calibrate Periodically function is usually sufficient to maintain the
wavelength and laser calibration of the Raman Rxn2.
A summary of the recommended calibration and verification sequence is as follows:
1. Internal calibration
2. Probe calibration
3. Probe verification
8.3.1 Internal calibration
Raman Rxn2 analyzers have internal calibration standards for spectrograph and laser wavelength. The internal
calibration options are:
• Automatic. If the instrument is already calibrated, this setting compares the current analyzer response to
calibration specifications and applies an algorithmic correction if it is minorly out of specification. This setting
also recalibrates if the spectrograph wavelength, laser wavelength, or both are out of specification. If the
analyzer is uncalibrated, this performs an alignment calibration, followed by a full wavelength calibration and
full laser wavelength calibration.
• Recalibrate X Axis. Forces full wavelength and laser calibrations without first checking whether the analyzer is
within spec.
• Recalibrate All. This setting causes the alignment calibration to be repeated prior to performing full
spectrograph wavelength and laser wavelength calibrations. Note that when Recalibrate All completes, the
intensity calibrations and verifications of all probes are invalidated.
Refer to the Calibration and verification section of the Raman RunTime Operating Instructions (BA02180C) for steps
to perform or set periodic internal calibrations.
8.3.2 Probe calibration
The sensitivity of the Raman Rxn2 varies with wavelength due to variations in the throughput of the optics and the
quantum efficiency of the CCD. The probe calibration function in Raman RunTime can be used to remove the effects
of this variation from measured spectra.
Probe calibration for Raman Rxn2 analyzers can be performed using a probe-specific calibration kit or the HCA
Raman calibration accessory. Refer to the applicable probe or optics manual to determine the appropriate calibration
accessory. Refer to the calibration accessory manual for details on how to calibrate the specific analyzer/probe
combination. Each channel must be probe-calibrated separately.
Probe calibration can be performed during active experiments, for example when a probe needs to be set up while
another probe is active. When a probe calibration is triggered, any acquisitions currently in progress are aborted
automatically, and the calibration proceeds. Upon completion of the calibration, active probes automatically resume
normal operation.
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