Chapter 3: Operation
Start the Laser
38 Hyperion Imaging System: User Guide
NOTE The area available for ablation is approximately 16,000 µm in the Y direction and
60,000 µm in the X direction.
9 The chamber automatically flushes for approximately 5 minutes, (unless the default
flushing time has been changed in the HTI Settings). Check the HTI Status Panel to
confirm that the chamber has been flushed (blue circle).
Start the Laser
The laser is automatically started when the system is started. To conserve the laser
between acquisitions without stopping plasma, click Stop Laser. The chamber will begin
flushing and the button toggles to Start Laser.
1 In the Toolbar click Start Laser to turn on the laser power. Check the HTI Status Panel
to confirm that the laser is on (blue circle).
NOTE See CyTOF Software v7.0 Help for procedures related to instrument control, tuning,
and data acquisition.
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