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Hyperion Imaging System User Guide
Troubleshooting
IMPORTANT If the symptoms below are not resolved using the information in the table,
contact
42TFluidigm Support42T.
Table 21. Troubleshooting tips for the Hyperion Imaging System.
Symptom Possible Cause Recommended Solution
Instrument Initialization Errors
Laser not turning on in software. Instrument not connected to power
source.
Click Start Laser in the software
Low helium supply pressure. Check helium pressure and change if
helium tank if required.
High chamber pressure reading in
the Status Panel window (>2 psi)..
Blocked Injector.
Use the recommended brush to
clean the injector. If pressure
remains high contact
42TFluidigm
Support
42T.
Stage will not move or moves
erratically.
XY start position not set correctly. Click Camera > Action > Home XY
Stage. If this does not resolve the
issue you may need to power-cycle
the instrument. Shut down the
software and then turn off the power
on the laser to re-establish correct
communication.
Ablation quality poor, blurry image,
no ions detected
Slide may be inserted incorrectly. Ensure that the sample is facing up.
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