Troubleshooting
Periodic Maintenance
Hyperion Imaging System User Guide
59
Symptom Possible Cause Recommended Solution
Coupling Tube Connection Errors
Lu(175) Mean Duals <500 (Tuning) Coupling tube not well-seated into
the ablation chamber or into the
Injector.
Verify coupling tube connections to
the injector and the ablation
chamber.
Coupling tube fitting and nuts are too
tight.
Check that coupling tube is correctly
connected. Nuts should be finger
tight.
Coupling tube not correctly
connected.
Reinstall coupling tube.
The injector/fitting may be pulled out
slightly because the tube is under
tension.
Check that the Injector and fitting are
correctly installed. The coupling tube
should be as straight as possible.
No ions in the Masses per Reading
window
Coupling tube not correctly
connected.
Check that coupling tube is correctly
connected. Nuts should be finger
tight.
Blocked coupling tube or blockage
in the ablation chamber lid.
Flush argon through the system by
adjusting the makeup gas flow to 1.0
L/min in the Control Panel > HTI
Setup > Chamber/Gases > Flushing
Makeup gas flow. Flush for 1 min.
Helios XY has not been tuned
correctly.
In the CyTOF Software click
AutoTune and click New.In the
General tab, under Subcalibrations,
check Coarse XY Optimization, Fine
XY Optimization, QC Report, Enable
HTI Current/Gases Calibration, and
Transients Calibration.
Detector voltage is incorrectly set. Manually adjust detector voltage in
the software. Refer to the CyTOF
Software v7.0 Help.
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