Troubleshooting
Periodic Maintenance
60 Hyperion Imaging System: User Guide
Symptom Possible Cause Recommended Solution
Wide transient width. O-ring seals on coupling tube
connections not correctly made.
Reinstall coupling tube. In the HTI
Status Panel check the chamber
pressure and the helium flow.
Poor signal The injector and fitting may be pulled
out a bit because the tube is under
tension.
Check that injector and fitting are
correctly installed. The tube should
be as straight as possible.
Laser Not Firing
Laser Not Firing
Laser interlock tripped Turn the laser off and then on in the
software. Contact
42TFluidigm Support42T
Tuning Errors
No ion signals Leak in chamber Check for gas leaks. Tighten fittings
and nuts on the coupling tube.
Gases are set incorrectly Makeup gas should be
approximately 0.8–1.0 L/min and
helium should be approximately
0.15–0.3 L/min. These values will
vary depending on the specific
Hyperion Imaging System. Contact
Fluidigm Support.
Ablation chamber door may be open. Close the door. Flush the gases
again.
Ablation chamber cover not properly
secured.
Contact 42TFluidigm Support42T.
Lu(175) Mean Dual counts <500
during tuning
Small fiber on one of the ends of, or
inside of the coupling tube, causing
turbulent gas flow.
Disconnect coupling tube. Inspect
with magnifying loupe and remove
any fibers found.
Injector not inserted correctly.
Check that the Injector is firmly
seated in the torch assembly, but
also that the Injector Sealer Cap is
tightly secured.
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