Troubleshooting
Periodic Maintenance
Hyperion Imaging System User Guide
61
Symptom Possible Cause Recommended Solution
AutoDV optimization performed with
abnormally high Xe131 (or alternate
isotope) levels.
Identify sources of leak and resolve.
Check the torch for correct seal.
Check coupling tube for damage. If
the issue persists contact Fluidigm
Support.
Lower-than-expected ion signals.
XY position on Helios is not
optimized.
Perform Pre-Calibration XY
Optimization and Fine XY
Optimization (see
42TAuto Tuning42T).
Makeup gas setting not optimized. Optimize the makeup gas settings
for better sensitivity. In the Data
Acquisition Settings window select
Makeup Gas in the drop-down menu
and perform transient analysis.
Current setting not optimized. Optimize the Gases/Current settings
for better sensitivity. In the Data
Acquisition Settings window select
Current in the drop-down menu and
perform transient analysis.
Transient width not within
acceptable range
Helium flow is not optimum. Check that the helium gas line is
connected correctly to the
communication panel of the
Hyperion Tissue Imager. Check that
the gas cylinder is open and the gas
line is connected correctly to the
cylinder.
Incorrect height of ablation stage. Contact 42TFluidigm Support42T.
Low mass peak means and signals. Sample not fully ablated. Select another region to ablate and
test the efficiency. If the ablation is
still not clean, power cycle the
instrument.
High CVs (>50%) during tuning
procedures. Multiple peaks in
Plotviewer.
High or low gain channel cables may
be switched on the Helios
preamplifier.
The gain channel cables are located
on the right side of Helios on the AC
panel. The Low Out 1 and the High
Out 2 cables may be switched.
Acquition Errors
Ablation is occurring, but there is no
or low ion signal.
Gas lines not connected. Check helium and argon gas line
connections.
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