Troubleshooting
Periodic Maintenance
62 Hyperion Imaging System: User Guide
Symptom Possible Cause Recommended Solution
Coupling tube not connected or
leaking.
Check coupling tube, and check that
the connections are tight.
Incorrect Ablation Power settings.
Determine the most efficient ablation
energy for the sample. Change the
Ablation Power settings.
No ablation on the slide.
Slide may have been inserted upside
down.
Check that the slide has been
inserted correctly. Contact
42TFluidigm
Support
42T.
No ablation on the slide.
Ablation energy has been set too
low.
Increase the Ablation energy in the
Acquisition window.
Slow/irregular stage movement
during ablation.
Check that the slide size is
compatible with the Hyperion
Imaging System. Power-cycle the
instrument and try ablating a new
area on the slide.
Other
Streaking in TIFF images Vibrations in the system. Check that the Hyperion Tissue
Imager has been fully lowered to the
ground. Check that the Helios
instrument castors have been locked
to minimize vibrations. Contact
42TFluidigm Support42T.
Uneven spacing of marks on the
glass slide.
Vibrations in the system. Check that the Hyperion Tissue
Imager has been fully lowered to the
ground. Check that the Helios
instrument castors have been locked
to minimize the vibrations. If this
continues to occur, Contact a
42TFluidigm Support42T.
Laser produces a thin/wavy ablation
pattern.
Laser has not sufficiently warmed up. Allow 10 minutes for laser to warm
up.
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