Prepare the waste tubing
CAUTION
Fasten the waste tubing. Make sure that tubing is securely fas-
tened to the waste ports W, W1, and W2.
Make sure that the waste tubing is prepared according to the instructions in Section 4.2.2
Install waste tubing, on page 46.
Prepare the outlet tubing
Connect tubing to the outlet ports of the outlet valve that are to be used during the run,
Out1 and/or Frac.
If no fraction collector is used, immerse the outlet tubing from Out1 in a suitable flask.
If a fraction collector is used, make sure that 400 mm tubing is connected between the
fraction collector and the Frac port on the outlet valve, and prepare the fraction collector
for a run. For more information, see the Fraction collector F9-C and F9-R Operating in-
structions.
Plug unused valve ports
It is recommended to plug all unused valve ports with stop plugs before starting a run.
See ÄKTA go User Manual for information about connectors.
CAUTION
Make sure there is a sample loop or a stop plug in place in the loop
positions in the injection valve to avoid liquid spurting out during
valve turns.
ÄKTA go Operating Instructions 29360951 AC 63
5 Prepare the system for a run
5.1 Prepare the flow paths
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