Column storage
If the column is not going to be used for a couple of days or longer, it must be placed in
a storage solution (20% ethanol) after a Column CIP run. Refer to the instructions for
your column for specific storage instructions. To place the column in a storage solution,
use the following procedure.
If the column is in a salt-containing buffer, equilibrate the column in water
before putting it in 20% ethanol, otherwise salt might precipitate in the column.
Note:
ActionStep
Place the inlet tubing in the storage solution (20% ethanol).1
In the Process Picture, click on Pump.2
Enter a flow rate of half the recommended flow rate of your column.3
Click on Timer and select Volume.4
Under Volume, enter 4 times the column volume. Click Start.5
When the run has finished, remove the column, plug the top and bottom of
the column, and place in a refrigerator.
6
System storage
If the system is not going to be used for a couple of days or longer, clean the system
using the System CIP method (see Section 7.2 Perform system Cleaning-In-Place (CIP),
on page 92) and then put the system in storage solution. Start by removing the cleaned
column from the system, see above. To put the system in storage, follow the steps below.
ActionStep
Clean the system using a System CIP method created for the system, see
Section 7.2 Perform system Cleaning-In-Place (CIP), on page 92.
1
Place all inlet tubing in storage solution (20% ethanol).2
In the Process Picture, click on Pump.3
Tick the Wash box for all inlet tubing in storage solution and click Pump
Wash.
4
If the system is equipped with a fraction collector, start a fractionation.5
Enter a flow rate of 10 mL/min.6
Click on Timer, select Volume, and enter 25 mL.7
88 ÄKTA go Operating Instructions 29360951 AC
6 Run a method
6.6 Procedures after the run
To Next Page
Loading...
Need help?
General
