ActionStep
In the Calibration dialog, choose Conductivity monitor - user calibration
in the Monitor to calibrate list.
8
Flush the conductivity flow cell twice with 25 to 30 mL distilled water, using
a syringe attached to the Luer-to-SNAP connector. Leave the syringe at-
tached to the connector so that solution does not drain from the flow cell.
9
Flush the conductivity flow cell with conductivity standard solution, using a
syringe attached to the Luer-to-SNAP connector, then wait until the conduc-
tivity reading is stable.
10
In the Calibration dialog box, enter the theoretical conductivity value at the
current conductivity temperature in the Enter theoretical conductivity
value input field.
11
•
If a certified conductivity standard solution is used, use the supplied
theoretical conductivity value.
•
If a manually prepared 1.00 M NaCl calibration solution is used, see the
graph for conductivity value at the current temperature Conductivity
values of 1.00 M NaCl, on page 107.
In the Calibration dialog, click Calibrate.12
Result: The new conductivity cell constant is displayed in the Conductivity
cell constant/cm box.
In the System Settings dialog box, select Conductivity:Cond temp compen-
sation and set the Compensation factor back to the desired value, default
2.1%. Click OK.
13
Reconnect the system tubing to the conductivity monitor.14
Factory calibration procedure
Follow the steps below to restore the conductivity cell constant to the factory default
value.
106 ÄKTA pilot 600 User Manual 29274559 AA
5 Maintenance
5.4 Calibration procedures
5.4.2 Calibrate the conductivity monitor
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