DescriptionSetting
Use this option to apply all available sample. Sample appli-
cation will stop when the External air sensor 1 detects air
in the sample inlet tubing. See Section 3.1 External air sen-
sors (optional), on page 30 for more information about ex-
ternal air sensors.
Note:
The option in Phase Properties only supports External air
sensor 1. Additional air sensors can be supported by editing
the text instruction in the Sample Application phase.
Inject all sample using
ext. air sensor 1
When this option is selected, the following options are
available
•
Enable air sensor after enables the air sensor when
the specified volume has been pumped. Use this option
if the sample inlet tubing has not been primed with
sample or buffer and may contain air. Enter a volume
larger than the volume of the inlet tubing.
•
Set maximum volume to sets a maximum limit on the
amount of sample applied.
This option fills the inlet tubing with sample by pumping
the specified volume of sample. Enter a volume larger than
the volume of the inlet tubing.
Prime sample inlet
with
When this option is selected, select Pump wash after
priming. This will flush the flow path up to the column valve
with buffer.
Note:
The flow path can be primed manually if desired, see Priming
the inlet tubing manually, on page 66.
This option pumps the specified volume of buffer directly
after the sample. Buffer is taken from the buffer inlet
specified in Method Settings. The recommended volume
is 5 to 10 times larger than the flow path volume up to the
column valve. If the Air Trap valve is set to In-line, the
minimum recommended volume is 300 mL.
Finalize sample injec-
tion
Set the air trap valve to In-line if there is a risk that air may
enter the inlet tubing. This applies, for example, if the inlet
tubing has not been primed, or if an external air sensor
close to the inlet valve port is used to minimize sample loss.
Air trap valve
ÄKTA pilot 600 User Manual 29274559 AA 65
4 Operation
4.1 Sample application
4.1.2 Apply sample from a system inlet
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