DescriptionSetting
If this option is selected, sample application will be inter-
rupted when the UV signal reaches the specified level. This
feature is typically used in adsorption chromatography, to
detect sample in the flowthrough.
Interrupt sample appli-
cation at UV
Settings specified here determine how flowthrough during
sample application will be collected. See Section 4.2 Frac-
tionation, on page 71 for details of fractionation settings.
Fractionation during sample application is generally rele-
vant only for adsorption chromatography.
Fractionation settings
Priming the inlet tubing
manually
The sample inlet tubing can be primed manually if desired, using manual instructions
or the Process Picture.
Follow the steps below to prime one sample inlet tubing manually using the Process
Picture.
ActionStep
Set the following modules:1
•
Inlet A to inlet used for sample (for example, A2).
•
Air trap to By-pass.
•
Mixer (if installed) to By-pass.
•
Column valve to Waste 2.
Result: The flow path is opened as shown.
66 ÄKTA pilot 600 User Manual 29274559 AA
4 Operation
4.1 Sample application
4.1.2 Apply sample from a system inlet
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