GE Analytical Instruments ©2006 DLM 14291 Rev. A
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LOW/PRESSURE CHARACTERISTICS OF BAG KITS.............................................................................................. 8-14
STABILITY OF NO IN MYLAR BAGS.................................................................................................................... 8-15
OFF-LINE VERSUS ON-LINE EXHALED NO MEASUREMENTS.................................................................................. 8-17
TESTING BAGS FOR PINHOLE LEAKS..................................................................................................................8-17
9. BREATH-BY-BREATH AND CHAMBER SAMPLING FOR EXHALED NITRIC OXIDE.......................... 9-1
BREATH-BY-BREATH MEASUREMENTS..................................................................................................................9-1
Spontaneously Breathing Subjects...................................................................................................... 9-1
Ventilated Subjects............................................................................................................................... 9-2
NOA SETUP..................................................................................................................................................... 9-3
NO/Pressure Offset................................................................................................................................9-3
Humidified Circuits................................................................................................................................ 9-3
CHAMBER SAMPLING..........................................................................................................................................9-4
NOA SETUP..................................................................................................................................................... 9-6
10. NASAL NITRIC OXIDE...................................................................................................................... 10-1
RECOMMENDED SETUP..................................................................................................................................... 10-1
PERFORMING THE MANEUVER........................................................................................................................... 10-2
NOA SETUP................................................................................................................................................... 10-3
11. INSTALLATION AND SETUP: LIQUID MEASUREMENTS..............................................................11-1
SUPPLIES........................................................................................................................................................ 11-1
Gases...................................................................................................................................................... 11-1
Reagents................................................................................................................................................ 11-1
Lab Equipment...................................................................................................................................... 11-1
SETUP OF PURGE VESSEL................................................................................................................................. 11-2
Connections of tubing to glassware.................................................................................................. 11-3
Procedure for Tightening Swagelok Fittings.................................................................................................11-4
DILUTION OF ANTI-FOAMING AGENT................................................................................................................. 11-7
NOA SETUP FOR LIQUID MEASUREMENTS..........................................................................................................11-8
DEPROTEINIZATION PROCEDURES.................................................................................................................... 11-10
Cold ethanol precipitation............................................................................................................... 11-11
Zinc Sulfate/Sodium Hydroxide precipitation.............................................................................. 11-11
12. MEASUREMENT OF NITRIC OXIDE AND NITRITE IN LIQUID SAMPLES....................................12-1
APPARATUS FOR NITRITE REDUCTION ...............................................................................................................12-1
PREPARATION OF THE NITRITE REDUCING AGENT ..............................................................................................12-2
ADJUSTMENT OF PURGE GAS FLOW RATE .........................................................................................................12-3
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