3 Operation
22
Monitor UV-900 User Manual 18-1120-05 Edition AF
3.11 UV cell calibration
The path length in t he UV flow cell might differ from the nominal length (1, 2, 5
respective 10 mm) which interferes the calculation of the protein concentration
in the eluate. T o achiev e normalized absorbance, the path length in the UV fl ow
cell must be calibrated.
It is recommended to calibrate a new UV flow cell before use. An old flow cell
can be calibrated whenever you think that a calibration is required.
Equipment required
To perform the calibration you need test solutions, syringes and accessories for
desired cell length:
• UV-900 1 mm Calibr. kit, no. 18-6324-01
• UV-900 2 mm Calibr. kit, no. 18-6324-02
• UV-900 5 mm Calibr. kit, no. 18-6324-04
• UV-900 10 mm Calibr. kit, no. 18-6324-05
To calculate the real cell length use the supplied Excel-file:
• UV-900 Cell Calibr. Excel-file, no. 18-6324-06
Removing an old calibration setting
If the instrument already has a calibrated cell length set that differs from the
nominal length, a warning message appears on the UV monitor display at
start-up. Another message, displaying the calibrated cell length, also appe ars.
The display toggles between these messages. The display automatically
returns to the main operating menu after about 45 seconds, or if pressing
OK
.
1 To remove an old calibration value, press
Esc
while the display toggles
between the two messages. The
Set UV Cell Length
menu appears.
Press
OK
.
2 At the
Set Calibr. Length
menu, set the cell length to
0
. Press
OK
.
Preparing the calibration
1 Remove any old calibration value by setting the cell length to 0 (see above ).
2 Unpack the UV Test kit.
3 Make sure that the Flow restrictor is connected in the flow path after the
UV flow cell.
4 Mount the Union Luer female/ 1/16
"
male, included in the test kit, in the
upper inlet of the UV flow cell.
Please Check UV Cell!
Press OK/Esc to cont.
Real UV Cell Length
(?,??mm, SN??????
Set UV Cell Length
(--,--mm, SN------)
Set Calibr. Length
(--,--mm) --.--
Union
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