3 Operation
24
Monitor UV-900 User Manual 18-1120-05 Edition AF
7 Remove the syringe.
8 Inject the othe r four test solutions in increa sing concentration or der. Use a
new syringe for each solution.
Note:
Air trapped in the UV cell causes inaccurate mea surements. T o avoid
introducing air into the ce ll, gently fill the Luer union up to t he edge
using test solution from the syringe that is next. Then insert the
syringe into the Luer union.
9 After each injection, wait for a stable absorbance value and enter the
measured values in the column
UV -900 Absorbance (AU)
in the UV-900
Cell Calibr. Excel-file.
10 When all absorbance values have been entered, the real cell length is
shown next to the
Real cell length
square.
Note:
The regressions coefficient should be larger than 0.999.
If not, one or several measured values are faulty.
Entering the new real length
1 Select menu
Set UV Cell Length
in the UV monitor. Press
OK
.
The
Set
Calibr. Length
menu appears. Press
OK
.
2 Enter the
Real cell length
value (from the Excel-file) and press
OK
.
3The
Set UV Cell Type
menu appears. Check the cell type value.
If the v alu e is corr e ct, turn the select ion dial clockwise to go to next menu.
If the value has to be changed, press
OK
to change it.
4 At the
Set Cell Serial No.
menu press
OK
.
5 Enter the serial numbe r of the cell
(
see
the label on the flow cell). Press
OK
.
3.12 Changing flow cell
The flow cell can be changed when require d, for example from 2 mm to 10 mm
when the sensitivit y of the mea surement must change due to a small amount
of sample being applied, or from a 10 mm to 2 mm when a lower sensitivity is
desired, due to output signal limitation.
See section
2.3 Installing the flow cell
.
3.13 Restart after power failure
If the power supply to the instrument is interrupted, the instrument
automatically restarts itself and displays the main operating window. All set
values are retained in the instrument but the instrument starts with the lamp
switched-off.
Set UV Cell Length
(--,--mm, SN------)
Set Calibr. Length
(--,--mm) --.--
Set UV Cell Type
(2 mm)
Set Cell Serial No.
(SN ------) ------
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