Hamilton Microlab STAR - Page 74

Microlab
®
STAR User Manual
Page 74 of 225 610766/02
An important issue is to select the FRQWUROOLQJVHTXHQFH. This is either the aspiration, or the
dispense sequence. As long as both sequences are of the same length, this selection does
not influence the pipetting (if no pipetting error occurs).
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But think of the situation, where the aspiration sequence comprises e.g. 8 tubes, and the
dispense sequence is the one of a 96-well plate. What to do now with the 88 remaining
positions within the plate? If the aspiration sequence is controlling, the dispense sequence is
reduced to the length of the aspiration sequence and the pipetting stops after dispensing into
the first 8 positions of the plate sequence. If the dispense sequence is controlling, the
aspiration sequence will be repeated until it reaches the length of the dispense sequence.
This results in multiple (12 for an 8-channel instrument) transfers from the same 8 tubes to fill
the complete plate. Then the (controlling) dispense sequence is finished and pipetting stops.
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The following table gives an overview of the different situations and examples. L is the length
of the sequence.
The 4 cases are illustrated in the following diagrams:
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Use:
-Copy 2 plates into 1 (after
another)
-With reloading of Seq(Disp):
Transfer available tubes to as
many plates as needed.
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OHQJWKRI6HT$VS
Use:
-Transfer a variable number (<96)
of tubes into a 96-well plate (left
partly filled).
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Use:
-Transfers from many tubes to a
plate and stops when plate is
filled.
-With reloading plate sequences:
Automatically fill as many plates
as needed with liquid from
tubes.
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Use:
-Transfer reagent from a container
(or, e.g., 8 tubes) to a complete
plate.
-Copy tubes repeatedly into
plate.
-With reload of aspiration sequence:
Automatically reload tubes until
plate is filled.
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