6-12 Echo Liquid Handler User Manual
4. Turn off the coupling fluid pump.
5. Return the coupling fluid pump direction to normal. Select C
OUPLING
PUMP DIRECTION Normal.
6. Unscrew the cap on the coupling fluid bottle and remove the bottle
from the bottle caddy.
Clean and refill the coupling fluid bottle
1. Clean the coupling fluid bottle and tubes.
a. Add Alconox or hand soap and water to the bottle.
b. Scrub the bottle and tubes with a plastic bristle bottle brush.
c. Thoroughly rinse the bottle and tubes to ensure there is no soapy
residue. Use distilled water for the final rinse.
d. Allow the bottle to dry, if possible, or alternate with another 1000 mL
bottle.
Note: If you see foam while refilling the bottle, rinse the bottle and
tubes again with hot tap water.
2. Inspect the bottle, bottle cap and seal ring for cracks, other damage, or
foreign material that would cause a vacuum leak. Replace if necessary.
3. Fill the coupling fluid bottle with 900 mL of distilled water and add
1drop (50μL) of algaecide.
Note: The ratio of algaecide to water is optimized for deterring the
growth of algae while minimizing residual moisture on the bottom of
the plate.
4. Return the cap and its attached tubing to the coupling fluid bottle.
5. Attach the cap securely to ensure a tight seal for vacuum (hand tighten)
and return the refilled coupling fluid bottle to the bottle caddy.
6. Reconnect the vacuum line to the Fluidics Interface Panel.
Caution: Make sure that the vacuum line is connected and working
correctly before turning the coupling fluid pump back on.
If the vacuum line is disconnected or the vacuum system is not operating
correctly, when the coupling fluid pump is turned on, the coupling fluid can
flood the bottom of the system.
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