Luminex 200 User Manual

Luminex 200 System User Manual
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The optics assembly consists of two lasers. One laser excites the dye
mixture inside the xMAP microspheres and the second laser excites
the fluorophore bound to the surface of the xMAP microspheres.
Avalanche photo diode detectors measure the excitation emission
intensities of the color coding classification dye mixtures inside the
xMAP microspheres and a photomultiplier tube detects the excitation
emission intensity of the reporter molecule bound to the surface of
the xMAP microspheres. High speed digital signal processors and
advanced computer algorithms provide analysis of the xMAP
microspheres as they are processed through the Luminex 200
analyzer. Results of the analyses are processed and provided in a
report format.
Hardware The Luminex 200 system includes the following hardware:
• Luminex 200 analyzer
• Luminex XYP instrument
• Luminex Sheath Delivery System (Luminex SD™)
• Power cables
• Two long sample probes
• Reservoir
•Shield
•Heater block
• Sheath fluid container
• Waste container
• Sheath fluid line
• Air line
• Sheath fluid intake line
• Communications: 1 serial communication cable
• Communications: 1 USB communication cable
• Communications: 1 CANBUS cable (short cable)
• Barcode reader
• Sample probe height alignment kit
xMAP Technology
Reagents
IS 2.3 • Classification calibration microspheres (CAL1)
• Reporter calibration microspheres (CAL2)
• Classification control microspheres (CON1)

Luminex 200 Specifications

General

Power Requirements	100-240 VAC, 50/60 Hz
Dynamic Range	3.5 logs
Number of Analytes	Up to 100
Software	xPONENT
Application	Immunoassays, Nucleic Acid Detection, Enzyme Assays
Technology	xMAP (Multi-Analyte Profiling)
Bead Types	Color-coded microspheres
Lasers	2 (532 nm and 635 nm)
Assay Types	Immunoassays, nucleic acid assays, enzyme assays
Sample Volume	12.5 - 50 µL
PMT Detectors	Yes