Luminex 200 User Manual

Luminex 200 System User Manual
38
Calibration and
Control Problems
Sample arm does not go down
smoothly.
The 96-well plate is incorrectly
seated in the XYP instrument.
Adjust the 96-well plate.
The 96-well plate is warped. Inspect the 96-well plate.
Replace it if it is warped.
The sample arm is misaligned. Readjust the sample arm
horizontal alignment.
The sample probe is bent. Remove the sample probe from
the Luminex 200 analyzer. Roll
it on a flat surface. If it does not
roll smoothly, replace it with a
new sample probe. Adjust the
sample probe height (see
page 18).
Symptom Possible problem Solution
Calibration is slow or fails. The calibration microspheres
are not fully suspended.
Vortex the calibration vials to
resuspend the microspheres.
Wrong lot number or target
values are entered in Update
CAL Targets dialog box.
Verify that correct lot number
and target values are used.
The system calibrators are in the
wrong well on the plate.
Verify that calibrators are in the
correct well.
Not enough calibrator
microspheres added to the well.
Add at least five drops of
calibrator microspheres to the
well. Hold the vial upside down
at a 90 degree angle to the plate
while dispensing them.
Calibrator lot is expired. Use a fresh bottle of calibrator
microspheres.
The sample probe height is
incorrect.
Adjust the sample probe height.
See page 18.
The sample probe is clogged. Clean the sample probe. See
page 20.

Luminex 200 Specifications

General

Power Requirements	100-240 VAC, 50/60 Hz
Dynamic Range	3.5 logs
Number of Analytes	Up to 100
Software	xPONENT
Application	Immunoassays, Nucleic Acid Detection, Enzyme Assays
Technology	xMAP (Multi-Analyte Profiling)
Bead Types	Color-coded microspheres
Lasers	2 (532 nm and 635 nm)
Assay Types	Immunoassays, nucleic acid assays, enzyme assays
Sample Volume	12.5 - 50 µL
PMT Detectors	Yes