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Luminex 200 User Manual

Luminex 200
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xMAP Technology Troubleshooting
39
There is a partial clog in the
system.
Clean the sample probe, then
flush the system. See page 20.
There is air in the system. Verify sample probe height. Run
three Prime commands, two
Alcohol Flush commands, then
three Washes with water.
The waste container is not
vented.
Check that the seal in the waste
container cap is dry and that the
waste container cap is vented.
The waste line was moved
during system operation,
resulting in unstable flow rate.
Verify that the waste line is not
moved during system operation.
Possible problem with the laser. View the calibration trend
report. Check for dramatic
changes in temperature, sheath
pressure, or voltages. If any of
these are evident on the report,
contact Technical Support.
Zero events collected during
calibration.
There is a problem with fluid
levels.
Check the sheath and waste
fluid levels. Verify that tubing
for both bottles is tightly
connected to the instrument.
Check that the waste bottle cap
is vented.
Laser-related issue. Verify fluid is moving through
the system by performing a
Wash, which causes fluid to go
out to waste. If no fluid is going
to waste, clean the sample
probe, then flush the system
(see page 20). If the issue does
not resolve, contact Technical
Support.
The waste container is not
vented.
Check that the seal in the waste
container cap is dry and that the
waste container cap is vented.
The Cheminert fitting is loose. Make sure that the Cheminert
fitting is tightened.
Analyzer fails Controls. The control microspheres are
not fully suspended.
Vortex the control vials to
resuspend the microspheres.

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Luminex 200 Specifications

General IconGeneral
Power Requirements100-240 VAC, 50/60 Hz
Dynamic Range3.5 logs
Number of AnalytesUp to 100
SoftwarexPONENT
ApplicationImmunoassays, Nucleic Acid Detection, Enzyme Assays
TechnologyxMAP (Multi-Analyte Profiling)
Bead TypesColor-coded microspheres
Lasers2 (532 nm and 635 nm)
Assay TypesImmunoassays, nucleic acid assays, enzyme assays
Sample Volume12.5 - 50 µL
PMT DetectorsYes