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Luminex 200 User Manual

Luminex 200
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xMAP Technology Troubleshooting
37
Sample Probe Problems with the sample probe can lead to fluid leaks and
pressurization problems, as well as inhibit sample acquisition.
Symptom Possible problem Solution
Sample probe leaks. The sample probe is clogged. Clean the sample probe. See
page 20.
Sample arm is stuck in the up
position.
The system isn’t properly
pressurized.
Check pressure settings. Ensure
that the sample probe is not
clogged and there are no leaks
in the syringe seal or syringe
valve.
Sample arm is stuck in the down
position.
The sample probe height is too
low, or the path to the well is
blocked.
DO NOT turn off the Luminex
XYP instrument.
1. Remove the blue light
housing from the analyzer.
2. Unscrew the Cheminert
fitting. The system monitor
changes from “Busy” to
“Running” and sample
acquisition continues. Click
Cancel to make adjustments
before continuing with the
rest of the samples.
3. If the sample arm does not
raise, save the data that has
been collected. Turn off the
analyzer, but keep the XYP
instrument on.
4. Exit the Luminex software.
5. Turn the analyzer back on
and restart the software.
6. Run a Wash with distilled
water to remove air from
the system.
7. Verify probe height.

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Luminex 200 Specifications

General IconGeneral
Power Requirements100-240 VAC, 50/60 Hz
Dynamic Range3.5 logs
Number of AnalytesUp to 100
SoftwarexPONENT
ApplicationImmunoassays, Nucleic Acid Detection, Enzyme Assays
TechnologyxMAP (Multi-Analyte Profiling)
Bead TypesColor-coded microspheres
Lasers2 (532 nm and 635 nm)
Assay TypesImmunoassays, nucleic acid assays, enzyme assays
Sample Volume12.5 - 50 µL
PMT DetectorsYes