Luminex 200 System User Manual
42
Bead Detail
Irregularities
Use these tools to assist in diagnosing system and kit-related
problems:
• system calibrators
•system controls
• assay standards
• assay controls
• error messages
Review calibration/control trend reports routinely to detect trends.
Use system xMAP control microspheres to check the success of the
system calibration and for troubleshooting purposes. If there is a
problem with your kit results, xMAP controls can help determine if
the problem is analyzer related. If Calibration and Controls are
successful, contact the kit manufacturer.
A normal bead detail display is shown below. It depicts a tight bead
population within a white region.
The xMAP microspheres are not
fully suspended.
Gently vortex the plate or
resuspend beads with a
multichannel pipettor to ensure
that the microspheres are
present in the solution.
You are using photobleached
microspheres.
Replace the microspheres with a
fresh batch.
There is an insufficient number
of beads in the sample.
Ensure that there are 2000-5000
beads per beadset per well.
The sample is too concentrated. Dilute concentrated biological
fluids, such as serum or plasma,
at least 1:5.
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