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Luminex 200 User Manual

Luminex 200
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Luminex 200 System User Manual
16
Air Intake Filter A replaceable air intake filter cleans the air used to pressurize sheath
fluid. This filter is enclosed behind an access door located on the
back of the Luminex 200 analyzer.
Syringe The syringe delivers a sample from the 96-well microtiter plate to the
cuvette.
Sheath Filter The sheath filter removes particles greater than ten microns in
diameter from the sheath fluid.
Air, Waste, and
Sheath Fluid
Connectors
The air, waste, and sheath connectors, located on the left side of the
analyzer, connect to the SD system and waste fluid containers using
clear tubing. The air connector is green, the sheath fluid connector is
blue, and the waste fluid connector is orange.
Luminex Sheath
Delivery System
For proper operation, place the Luminex SD system at the same level
as the base of the Luminex XYP instrument. Do not put it on top of
the Luminex 200 analyzer. If you are not using the SD system, sheath
fluid levels must be monitored manually. Check the sheath fluid level
before starting a run or procedure.
Waste Fluid
Container
The waste fluid container receives waste from the system. The waste
container should not be placed on top of the instrument. Ensure
that the waste tubing is not elevated above the level of the
Luminex 200 analyzer at any point. Contact Luminex Technical
Support before you relocate the waste fluid container. To maintain a
stable flow rate, do not move the waste line or container during
system operation.
Optical The optical system consists of the optical assembly and the excitation
lasers. The optical assemblies do not require manual adjustment by
the user.
xMAP Technology
Reagents
The xMAP technology reagent system consists of classification
calibration microspheres, reporter calibration microspheres,
classification control microspheres, and reporter control
microspheres.
Warning: If biological samples have been tested with the sys-
tem, use your standard laboratory safety practices.
Warning: Waste levels must be manually monitored. Do not
allow the waste container to overflow.

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Luminex 200 Specifications

General IconGeneral
Power Requirements100-240 VAC, 50/60 Hz
Dynamic Range3.5 logs
Number of AnalytesUp to 100
SoftwarexPONENT
ApplicationImmunoassays, Nucleic Acid Detection, Enzyme Assays
TechnologyxMAP (Multi-Analyte Profiling)
Bead TypesColor-coded microspheres
Lasers2 (532 nm and 635 nm)
Assay TypesImmunoassays, nucleic acid assays, enzyme assays
Sample Volume12.5 - 50 µL
PMT DetectorsYes