Luminex 200 System User Manual
10
Accuracy and Precision • Sample uptake volume: ± 5%
• Classification of xMAP microspheres: > 80%
• Misclassification of xMAP microspheres: 2 % - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
• Temperature control: 0°C to + 2°C of target
• Internal sample carry over: < 0.9%
• Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
Sensitivity • Detect 1000 fluorochromes phycoerythrin (PE) per xMAP
microsphere
• Reporter channel dynamic range: 3.5 decades of detection
Capacity The specifications below reflect minimum capacity values:
• Analyze multiple 96-well plates per batch
• Analyze multiple assay templates per plate
• Distinguish a minimum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
• Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
• Sample core: 15-20 µm core at 1 µL/sec. sample inject rate
• Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
• Automatic sampling from a 96-well plate
• Start sampling from any well position
• Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
• Microtiter plates with 96 wells must be compatible with the
Luminex XYP instrument plate holder. The following microtiter
plate types are compatible with the Luminex XYP instrument
plate holder: flatbottom, conical, round, filter bottom, half plates,
overall height no more than 0.75” (19 mm), any color.
• Microtiter plates with 96 wells must be compatible with
Luminex XYP instrument heater block temperature from 35°C to
55°C (95°F to 131°F) when performing heated assays and using
the heater block.
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