PROGRAMMING AND OPERATION
17
Listed below are some programming considerations for the
mix operation:
n If the program begins a mix cycle from zero position, it will
complete the cycle with an automatic blowout, then
display "ZERO." Press the trigger to return the pistons to
zero position.
n If the program begins a mix cycle above zero position
(volume is greater than zero), the fluid is dispensed, and
the mix cycle begins. The mix cycle will end at the same
volume it started with. Also, the mix operation will remain
in the display so it can be repeated (useful for serial
dilutions). To end the mix operation, press the [Purge]
key, then press the trigger.
Purge. The [Purge] key programs the pipettor to purge the
fluid. This step can be entered as a program step, or it can be
pressed anytime while pipetting to terminate the program and
dispense the remaining liquid.
Note: If the program ends at a volume greater than zero, and
the last step is not a mix cycle, the "Purge" prompt
automatically appears. Also, the pipettor emits a beep tone to
warn you that some fluid remains. Press the trigger to
dispense the remaining fluid and return to the beginning of the
program.
Examples of pipetting programs with different mix steps are
shown in Section C.4, Programming Examples.
3. Enter volume. Enter the desired volume for [Fill], [Disp], or
[Mix] by pressing the up s or down t side of the [Vol] key. If
the [Vol] key is pressed and released quickly, the volume
changes by its smallest increment of measure (for example,
0.5 µl for the 250 µl pipettor); if the [Vol] key is pressed and
held, then the volume change occurs with larger increments
(see Table C.1, Default Programming Volumes, for volume
increment).
Note: The [Purge] key does not use a volume; therefore, if
you have entered a purge step, continue to the next step.
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