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• When the dry-out cycle is finished, the
pump stops, and the switch light stops
blinking; firmly press the filtration unit lid
down to lock it
Figure 18: filtration unit lid firmly pressed down
with the palm to lock
• Tilt the filtration unit first, then remove it
from the pump head
Figure 19: Filtration unit tilted for removal
• Optional: inspect underneath the
membrane to verify that it has a convex
shape
Figure 20: checking of the membrane convex
shape
Note: a convex membrane shape shows
that there is no leakage or pass-through
(membrane and seal integrity). In
addition, it helps in removing bubbles
or folds between the membrane and the
agar after membrane transfer.
• Open the pre-opened media plate with
one hand
Note: Be careful not to touch the agar
while removing the media plate cover.
• Place the filtration unit on the media
plate
• Using the thumb and index finger of each
hand, push down the funnel’s lid edges
vertically ensuring a central contact
between the membrane and the agar.
Make sure the filtration unit is firmly
pressed down against the media plate.
Figure 21: optimal handling for pushing down the
filtration unit to the media plate
Note: pushing down the filtration unit
vertically with four fingers ensures that
the membrane has full contact with the
agar without forming folds or bubbles.
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