Millipore Milliflex Oasis - Milliflex Cassettes; Prefilled Milliflex Cassette Types; Empty Cassette Preparation Guide

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8. Milliflex
®
cassettes
These products (older generation) can be
used instead of Milliflex Oasis
®
media plates.
These products do NOT have the following
features:
• Traceability stickers
• Traceability labels
• Color coding
• Stacking and locking features
Prefilled Milliflex
®
cassettes
Description:
Milliex
®
Cassette Sabouraud Dextrose Agar
(w/Chloramphenicol)
Milliex
®
Cassette m-Endo Agar for the detection
of coliforms
Milliex
®
Cassette PCA Agar for the detection
of bacteria in water
Milliex
®
Cassette Cetrimide Agar with Naladixic Acid
for detection of P. aeruginosa
Milliex
®
Cassette TSA and lecithin agar for the
detection of heterotrophic microorganisms
Milliex
®
Cassette HPC agar media, for detection
of stressed heterotrophic plate count bacteria
Milliex
®
Cassette KF Strep Agar for the detection
of fecal streptococci
Milliex
®
Cassette PIA Agar for the detection and
recovery of P. aeruginosa
Milliex
®
Cassette MacConkey agar for detection
of lactose fermenting bacteria and coliforms
Milliflex
®
empty cassettes
Empty cassettes are available for those
users who would like to prepare their own
culture media.
Milliflex
®
empty cassette for agar media
(item MXSMC0120)
• Prepare the agar medium for dispensing,
and have a suitable refilling dispense
syringe or pipette ready. Liquefy the solid
agar medium and maintain at less than
60 °C.
• Remove the frosted top cover of the
cassette.
1
• Use a dispensing syringe or pipette to add
9.5–10 mL of agar into the center of the
cassette.
2
Note: Do not add more than 10 mL of agar
to the cassette to avoid spilling media into
the internal gutter.
• Rest the cover back over the cassette,
without pushing it into place, and allow
the agar to solidify. Replace the cover by
pushing it into place.
3
Note: Do not close the cassette until the
agar solidifies.
1
2
3
Figure 48: steps to prepare a solid medium cassette
Remove the
top cover
Add 10 mL of
agar medium (max)
Replace the cover
and wait until the
agar solidies
Top
Cover
Bottom
Cover
Dispensing
Syringe