Page 24 of 32
7. Culture media growth promotion test
The culture media growth promotion
test, as described in the fully harmonized
pharmacopoeias (USP <61>, EP 2.6.12,
JP 4.05 part 1), can be performed using
the growth promotion accessory (item
MMRECVERY), which is a Milliflex Oasis
®
funnel without membrane.
This accessory allows for good inoculum
spreading and includes a lid to close the
media plate.
This section describes the use of this
accessory:
• Obtain and prepare the appropriate
microbiological cultures according to
internal SOPs or pharmacopeia guidelines.
Concentrations should be less than or
equal to 100 Colony Forming Units (CFU)
per sample
• Place the media plates in the working area
• Place a tray of Milliflex Oasis
®
funnels
without membranes (item MMRECVERY) in
the working area
• Remove the media plate cover
• Remove the funnel assembly support
• Place the funnel assembly on the
media plate
• Make sure the funnel assembly is firmly
pressed down against the media plate
• Remove the funnel by pinching it while
maintaining the base with the other hand
• Tilt the funnel lid sideways to remove it
and store it under the workspace. Discard
the unused funnel
• Dispense the inoculum sample onto the
surface of the media plate assembly
and use a sterile spreader to spread the
inoculum on the medium
Note: it is recommended to spread no more
than 50 µL on a media plate assembly. For
method consistency, the same volume must
be spread on the control Petri plate.
• Cover the spiked media plate assembly
with the funnel lid
• Incubate the media plate assembly at the
specified temperature and time
Note: Incubate the media plate assembly
with the lid facing down.
To Next Page
Loading...
