Chapter 3 Individual Operations
86
Individual
Operations
Contents of the “microscope state” display are as follows.
Microscope State Display Contents
Objective
[
__000x]: Blank: Achromat
[
AT000x]: Apo TIRF
[
P _ 000x]: Plan
[
PA000x]: Plan Apo(TIRF/VC)
[
PT000x]: Plan Apo TIRF
[
PV000x]: Plan Apo VC
[
PF000x]: Plan Fluor
[
PU000x]: Plan UW
[
SF000x]: S Fluor
[
SP000x]: S Plan Fluor
[
HM000x]: HMC
[
OT000x]: Others
[
F _ 000x]: Fluor
[
EP000x]: E Plan
[
NA000x]: NIR Apo
[
PI000x]: Plan Apo IR
[
A _ 000x]: Apo(λ/LAMBDA)
Optical path switching [BINO]: Observation port (binocular)
[
FRONT]: Tube adapter
[
REAR]: Rear port
Z axis position [Z:_ 00000.000um]
[
Z:-00000.000um]
+ direction: Stage rises
- direction: Stage lowers
Status of the 1st
epi-fluorescence cube turret
[
UV-2A] Name of the filter cube in the optical path of the motorized/intelligent filter cube
(1st layer) (Example)
Status of the 2nd
epi-fluorescence cube turret
[
UV-2B] Name of the filter cube in the optical path of the motorized/intelligent filter cube
(2nd layer) (Example)
Motorized XY stage position [X:_ 000000.000um]: Motorized XY stage X coordinate
[
Y:_ 000000.000um]: Motorized XY stage Y coordinate
EPI motorized shutter status [EPI:O]: EPI motorized shutter open (OPEN)
[
EPI:C]: EPI motorized shutter closed (CLOSE)
Motorized barrier filter wheel [BA000-000]: Barrier filter name
Motorized excitation filter wheel [EX000-000]: Excitation filter name
Motorized HG precentered fiber
illuminator's ND status/built-in
shutter status
[
ND00
'
O ]: ND value, shutter open (OPEN)
[
ND00
'
C ]: ND value, shutter closed (CLOSE)
Aperture diaphragm status [AS00.0mm]: Aperture diaphragm diameter
Field diaphragm status [FS00.0mm]: Field diaphragm diameter
Zoom magnification [ZM:0.0x]: Zoom magnification
Motorized ND filter wheel status [ND00.0%]: ND value
Condenser status [OPEN_ ]: Empty (bright-field)
[
2/4x_ ]: 2-4x auxiliary lens
[
N1___]: DIC module
[
N2___]: DIC module
[
NR___]: DIC module
[
Ph1__]: PH module
[
Ph2__]: PH module
[
Ph3__]: PH module
[
DF___]: Dark-field module