Chapter 2 Microscopy Operations
34
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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Close the shutter and block the illumination path.
Press the [FL SHUTTER] button and close the shutter in
the motorized epi-fluorescence cube turret. The shutter
is closed when the shutter button is lit.
Shutter
The shutter blocks illumination.
If the specimen is continuously exposed to the strong
light of the mercury lamp, it may become damaged or
decolorized.
Make sure you closed the shutter when you suspend the
microscopy or when pausing epi-fluorescence
microscopy to perform microscopy using diascopic light.
Be sure to get into the habit of performing this operation.
ND
8
ND
32
OUT
IN
NCB
11
Closing shutter
PF__2x____Z:_____0.000um
UV-2B
'
C ____ND_ 1
'
C __EPI:C
C: Shutter closed
Shutter blocking epi-fluorescence light
The shutter is built into the epi-fluorescence cube
turret and is also equipped on the HG precentered
fiber illuminator. Also, an EPI motorized shutter can
be attached to the microscope. Epi-fluorescence
illumination can be blocked by closing one of the
shutters. (See Chapter 3 “16.3 Protecting the Sample
and Preventing It from Decoloration (Using the
Shutter)”.)
[SHUTTER FL] button: Open/close the
shutter in the motorized epi-fluorescence
cube turret
Label of the button changes indicating the
current open/close status.
[OPEN]: open, [CLOSED]: closed
When you are using intelligent
epi-fluorescence cube turret, the [SHUTTER
FL] button does not appear. Therefore, it is not
possible to determine the shutter open/closed
status on DS-L3.
Lit
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