Chapter 2 Microscopy Operations
37
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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7
Open the shutter.
Press the [FL SHUTTER] button and open the shutter in
the motorized epi-fluorescence cube turret which is
closed in procedure 2.
When the shutter is opened, the button illumination will
turn off.
ND
8
ND
32
OUT
IN
NCB
11
Opening the shutter
PF__2x____Z:_____0.000um
UV-2B
'
O ____ND_ 1
'
C __EPI:C
O: Shutter opened
[SHUTTER FL] button: Open/close the
shutter in the motorized epi-fluorescence
cube turret
Other shutters
If the shutter of the HG precentered fiber illuminator
or EPI motorized shutter is closed, be sure to open
these shutters as well.
Label of the button changes indicating the
current open/close status.
[OPEN]: open, [CLOSED]: closed
When you are using intelligent
epi-fluorescence cube turret, the [SHUTTER
FL] button does not appear. Therefore, it is not
possible to determine the shutter open/closed
status on DS-L3.
Light shielding plate on the
epi-fluorescence cube turret
The light shielding plate protects the observer's eyes
from reflected ultraviolet light, which is originally
emitted from the objective at the specimen.
1
2
A
λ
F. S TOP
2
3
4
5
6
50
60
70
Light shielding
plate
Off
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