Nikon ECLIPSE Ni-E User Manual

Chapter 2 Microscopy Operations
44
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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(4) When differential interference contrast microscopy is
finished, go to epi-fluorescence microscopy.
• Press the dia-illumination ON/OFF switch to turn of
f
the lamp and make the diascopic image disappear.
Dia-illumination lamp OFF
• Rotate the epi-fluorescence cube turret to bring the
desired filter cube into the optical path.
ND
8
ND
32
OUT
IN
NCB
11
Bringing the filter cube into the optical path
• Remove the FL/DIC analyzer slider from optical
path.
A
F. S TOP
2
3
4
5
6
50
60
70
Removing the FL/DIC analyzer slider from optical path
• Fully open the field diaphragm and aperture
diaphragm of the epi-fluorescence attachment.
F. S TO P
A. STOP
EX. ADJ
Fully opening field and aperture diaphragms
(5) Turn on the power of the HG precentered fiber
illuminator and continue from procedure 6 in the
epi-fluorescence microscopy procedure.
NI-FA FL/DIC
A
nalyzer Slide
r
A
perture
diaphragm lever
Field diaphragm
leve
r

Nikon ECLIPSE Ni-E Specifications

General

Brand	Nikon
Model	ECLIPSE Ni-E
Category	Microscope
Language	English