Nikon ECLIPSE Ni-E User Manual

Chapter 2 Microscopy Operations
48
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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Concurrent Use of Epi-Fluorescence/Phase Contrast Microscopy
By bringing the following optical elements into the optical
path, epi-fluorescence and phase contrast can be
simultaneously observed by microscope:
Filter cube for the epi-fluorescence cube turret
Ph objective
PH module for the condenser
• If the diascopic image is not shown under
epi-fluorescence microscopy, press the
dia-illumination ON/OFF switch to turn on the lamp.
• Remove the GIF filter if it is installed on the field
lens.
IN
OUT
1
2
A
λ
F. ST OP
A
EX.
2
3
4
5
6
Concurrent use of epi-fluorescence/phase contrast
microscopy
• Adjust the fluorescent image brightness with ND
filters in the epi-fluorescence attachment, and
phase contrast image brightness using ND filters in
the main body. Dia-illumination should be
sufficiently dimmed using the ND filter.
ND4
ND8
ND16
Brightness adjustment of fluorescent image
ND
8
ND
32
OUT
IN
NCB
11
Adjusting brightness of phase contrast image
Ph objective
Filter cube
PH module

Nikon ECLIPSE Ni-E Specifications

General

Brand	Nikon
Model	ECLIPSE Ni-E
Category	Microscope
Language	English