Chapter 2 Microscopy Operations
47
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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• Rotate the epi-fluorescence cube turret to bring the
desired filter cube into the optical path.
ND
8
ND
32
OUT
IN
NCB
11
Bringing the filter cube into the optical path
• Fully open the field diaphragm and aperture
diaphragm of the epi-fluorescence attachment.
F. S TO P
A. STOP
EX. ADJ
Fully opening field and aperture diaphragms
(4) Turn on the power of the HG precentered fiber
illuminator and continue from procedure 6 in the
epi-fluorescence microscopy procedure.
perture
diaphragm lever
Field diaphragm
leve
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