Chapter 2 Microscopy Operations
55
Differential
Interference Contrast
Microscop
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
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9
Place a specimen on the stage, and move the stage to bring the target into view.
(1) Open the claw of the specimen holder's moving part
and place the specimen onto the stage, gently stowing
the claw back to fix the specimen.
(2) Rotate the stage X/Y knobs to move the stage and
bring the target into the optical path. (See Chapter 3 “5
Bringing the Target into the Optical Path (Horizontal
Stage Movement, Rotation)”.)
(So that the sample sealed under the cover glass will
be lighted.)
Non-fluorescent, distortion-free slide glass
Use clean, non-fluorescent, distortion-free slides and
cover glasses.
Setting specimen
Motorized horizontal stage movement
When using a motorized XY stage, use the ergo or
joystick controller to control the horizontal stage
movement. (See Chapter 3 “5.2 Operating the
Motorized XY Stage”.)
IN
ND
8
ND
32
NCB
11
OUT
Knob rotation and stage movement direction
Cover glass on top
Stage Y knob
Stage X knob
Y direction
X direction
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