Chapter 2 Microscopy Operations
56
Differential
Interference Contrast
Microscopy
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
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10
Focus on the sample.
(1) Looking through the eyepiece, adjust the brightness for
optimum brightness by rotating the dia-illumination
brightness control knob.
(2) Looking through the eyepiece, focus on the sample by
rotating in the order of the coarse focus knob and then
the fine focus knob. (See Chapter 3 “4.1 Proper
Focusing Procedure”.)
Z-RESET
The Z-axis coordinate value can be reset to zero.
This is useful when you wish to use the current
position as the reference position in adjusting the
focus. (See Chapter 3 “4.3 Resetting the Z-axis
Coordinate”.)
Refocusing
This operation moves the Z-axis position to the
retracting position or returns it to the original position.
(See Chapter 3 “4.4 Refocusing”.)
Brightness adjustment
Brightness adjustment
Focusing using the focus knob
PA_ 10x____Z:__1234.567um
OPEN__AS30.0mm__FS30.0mm
Z-axis coordinate
Note on controlling the focus knobs
Avoid the following action, which can cause equipment
malfunction.
• Rotating the right and left focus knobs in opposite
directions.
Do not turn the focus knobs in opposite direction!
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