Chapter 2 Microscopy Operations
62
Differential
Interference Contrast
Microscopy
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
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20
Adjust the field diaphragm.
Press the DIA field diaphragm button to adjust the field
diaphragm so that it circumscribes the field of view.
Size of the field diaphragm
Normally, the field diaphragm should be adjusted so
that it circumscribes the field of view. Excessively
opening the field diaphragm will result in stray light
entering the field of view, generating flare and
reducing the image contrast. It will also cause the
specimen to become decolorized over a wider area.
Adjustment timing for the field diaphragm
Be sure to adjust the field diaphragm each time you
change the objective.
ND
8
ND
32
OUT
IN
NCB
11
Adjusting field diaphragm
PA_ 40x____Z:__1235.555um
N1___AS12.3mm___FS25.0mm
Appropriate size of the field diaphragm
[F. STOP] button,
sub screen: DIA field diaphragm adjustment
Field diaphragm
Field of view
Circumscribe around the field of view.
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