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Olympus CKX53 - Summary of Fluorescence Observation Procedures

Olympus CKX53
60 pages
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48
(Operation portion) (Page)
8Field iris diaphragm lever (page 21)
1Main switch (page 15)
(page 11)
4Revolving nosepiece (page 16)
5Filter slider for reflected light illumination (page 16)
³Fluorescence mirror unit selection lever (page 16)
(Attenuator filter)
5Filter slider for reflected light illumination (empty hole)
(page 16)
When performing the fluorescence observation, be sure to engage the umbra shield in the light path.
· If you take a short break during observation, engage the shutter for reflected light illumination of ƒ filter slider
for reflected light illumination in the light path
· The numbers assigned to operating portions indicate operating portions described in "2-2 Fluorescence set"
(page 9).
CAUTION
TIP
6Coarse focusing knob (page 16)
7Fine focusing knob (page 16)
9-2 Summary of fluorescence observation procedures
9X-axis knob (page 16)
aY-axis knob (page 16)
(page 47)
2Umbra shield (page 15)
Place the specimen on the stage.
Engage the fluorescence mirror unit suitable for the
specimen in the light path.
If necessary, engage the dark
filter in the light path to adjust
the brightness.
Adjust the field iris diaphragm.
Attach the objectives suitable for observation methods.
Centering of the mercury burner (when using the mercury lamp housing U-LH100HG)
Turn ON the main switch of the power supply unit and
wait until the arc image is stabilized (5 to 10 minutes).
Engage the objective in the light path, open the shutter for
reflected light illumination and bring the specimen into focus.
Preparation
Observation Procedures
Move the specimen.
Find the position of the specimen to perform the fluorescence observation
in the phase contrast observation or the bright field observation.
Start observation.
Engage the umbra shield (ambient light shielding plate)
in the light path.

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