Olympus Fluoview-1000 User Manual

.............................................................................................................FV-1000 Optical Specifications 48
.................................................................................................................................................No Image! 50
..................................................................................................................................Start-up problems. 50
..............................................................................................................................Scans, but no image. 50
..............................................................................The confocal image appears unevenly illuminated: 50
...................................................The epifluorescent or tungsten images appear unevenly illuminated: 50
Figures
Figure 1. Main Fluoview window. 6
Figure 2. Enlarged view of Fluoview windows. 8
Figure 3. Microscope Controller Window 9
Figure 4. Image Acquisition Window – Basic controls for Finding the Image 11
Figure 5. Channel Settings for the Photomultiplier Tube (PMT) 14
Figure 6. The Scan Mode and Scan Size settings of the Acquisition Setting Window. 16
Figure 7. Selecting laser lines and setting laser power 15
Figure 8. Scan Controls for Zoom, Pan and Rotation 19
Figure 9. Sequential Imaging Control 20
Figure 9A. Sequential Imaging Control 20
Figure 10. Z-Series Control Window 24
Figure 11. Z-Series Control Window 24
Figure 12. DIC and Focus Controls, Lower Half of the Microscope 40
Figure 13. Condenser and DIC Controls 42
Figure 14. Tilting the condenser. 43
Figure 15. The Stimulus Setting window for photoactivation 45
Figure 16. Photobleaching an ROI. 46
Figure 17. The toolbar item for control over display color 47
Figure 18. Fluoview appearance in Darkroom Color 47
Tables
Table 1. Mirror Buttons 10
Table 2. Objective Lenses Available 12
Table 3. Scan Speed and Acquisition Time 14
Table 4. Optimal Z-Step Values 37
Table 5. Pixel Size (µm) versus Box Size and Magnification at Zoom = 1 39
Table 6. Dichroic Mirrors and Barrier Filters 38
Table 7. Laser Lines on the FV-1000 38
Table 8. Index of Refraction for Common Mounting Materials 49
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 4

Olympus Fluoview-1000 Specifications

General

Brand	Olympus
Model	Fluoview-1000
Category	Microscope
Language	English